Improving Microscopy

Dr. Christopher Raymond uses mathematical and experimental modeling to improve labeling techniques for biological samples on the electron microscope.

Mathematical and experimental modeling of immunocolloid labeling techniques for electron microscopy is the focus of research by Christopher S. Raymond, assistant professor of mathematical sciences. The goal is to develop a new labeling technology that will allow investigators to rapidly and reliably identify and localize multiple molecular species in a single specimen.


Immunocolloid labeling is a technique for high resolution studies of biological structure and ultrastructure. Nanoscale metal particles joined to antibodies or other biomolecules scatter electrons efficiently allowing the biomolecules to be discerned under an electron microscope. Immunocolloid labeling has been used for a wide variety of purposes, including the detection of certain viruses and bacteria and direct observation of the development of blood clots. In research funded by the NIH, Dr. Raymond aims to gain a better understanding of the labeling process to optimize the choice of experimental conditions and maximize the efficiency and accuracy of labeling.